Application

This Milli-Mark Anti-pan Histone-FITC Antibody, clone H11-4 is validated for use in FC for the detection of pan Histone.

General description

There are 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. The five major classes of histones are H1/H5, H2A, H2B, H3, and H4. Histones H2A, H2B, H3 and H4 are known as the core histones, while histones H1 and H5 are known as the linker histones. Histones feature a main globular domain and a long N terminal tail which are involved with the structure of the nucleosomes of the ′beads on a string′ structure. They are the chief protein components of chromatin, acting as spools around which DNA winds, and play a role in gene regulation.

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Quality

Evaluated by flow cytometry using HeLa cells.

Specificity

Due to the highly conserved nature of histones, this antibody is predicted to have wide species reactivity.

Target description

Antibody recognizes histones H1, H2A, H2B, H3, and H4. Calculated molecular weight for these histones range from 14 to 21 kDa